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ulbp 1  (R&D Systems)


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    R&D Systems ulbp 1
    Ulbp 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ulbp+1+pe+conjugated+antibody/Human+ULBP-1+PE-conjugated+Antibody/pm41826286-310-30-54
    Average 93 stars, based on 46 article reviews
    ulbp 1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: NKp44-based chimeric antigen receptor effectively redirects primary T cells against synovial sarcoma
    Article Snippet: .. Expression of NKG2D ligands was detected using PE-conjugated MICA/B monoclonal antibody (Biolegend, CA, USA), human ULBP-1 PE-conjugated antibody (R&D systems), human ULBP-2 PE-conjugated antibody (R&D Systems), and human ULBP3 antibody (R&D Systems), followed by staining with PE-conjugated F(ab’)2 fragment goat anti-mouse IgG secondary antibodies (Jackson ImmunoResearch). .. Purified mouse IgG2a, κ isotype antibody (BioLegend) was used as the control.

    Staining:

    Article Title: NKp44-based chimeric antigen receptor effectively redirects primary T cells against synovial sarcoma
    Article Snippet: .. Expression of NKG2D ligands was detected using PE-conjugated MICA/B monoclonal antibody (Biolegend, CA, USA), human ULBP-1 PE-conjugated antibody (R&D systems), human ULBP-2 PE-conjugated antibody (R&D Systems), and human ULBP3 antibody (R&D Systems), followed by staining with PE-conjugated F(ab’)2 fragment goat anti-mouse IgG secondary antibodies (Jackson ImmunoResearch). .. Purified mouse IgG2a, κ isotype antibody (BioLegend) was used as the control.

    Article Title: Exploring the correlation and mechanism of natural killer cell cytotoxic sensitivity against gastric cancer
    Article Snippet: Data were analyzed using ACEA Novo Express software (NovoExpress1.1.0, ACEA Biosciences Inc., San Diego, CA, USA). .. The following antibodies were used to stain single cell suspensions with the dilution ratio of 5 μL per million cells in 100 μL staining volume: FITC anti-human CD3 (UCHT1), APC anti-human CD56 (HCD56), PE anti-human CD314 (NKG2D) (1D11), APC anti-human CD340 (erbB2/HER-2) (24D2), APC Mouse IgG1, κ isotype Ctrl (MOPC-21), PE Mouse IgG1, κ isotype Ctrl (MOPC-21), PE anti-human MICA/MICB (6D4), PE anti-human CD336 (NKp44) (P44-8), PE anti-human CD337 (NKp30) (P30-15), and PE anti-human CD16 (3G8) (BioLegend, Inc., San Diego, CA, USA), and Human ULBP-1 PE-conjugated Antibody (170818), Human ULBP-2/5/6 PE-conjugated Antibody (165903), and Human ULBP-3 PE-conjugated Antibody (166510) (R&D System, Minneapolis, MN, USA). .. The YSH-LV001 vector (Ubigene Biosciences, Guangzhou City, China) was used to construct a specific CD56 shRNA-expressing plasmid.

    other:

    Article Title: Antitumor activity of NKG2D CAR-T cells against human colorectal cancer cells in vitro and in vivo
    Article Snippet: The expression of NKG2D, CD4 and CD8 on CAR-T cells was directly detected with human NKG2D/CD314 APC-conjugated antibody, CD4 PerCP-Cy5.5-conjugated antibody and CD8 PerCP-Cy5.5-conjugated antibody (all BD, San Diego, CA, USA).

    Single Cell:

    Article Title: Exploring the correlation and mechanism of natural killer cell cytotoxic sensitivity against gastric cancer
    Article Snippet: Data were analyzed using ACEA Novo Express software (NovoExpress1.1.0, ACEA Biosciences Inc., San Diego, CA, USA). .. The following antibodies were used to stain single cell suspensions with the dilution ratio of 5 μL per million cells in 100 μL staining volume: FITC anti-human CD3 (UCHT1), APC anti-human CD56 (HCD56), PE anti-human CD314 (NKG2D) (1D11), APC anti-human CD340 (erbB2/HER-2) (24D2), APC Mouse IgG1, κ isotype Ctrl (MOPC-21), PE Mouse IgG1, κ isotype Ctrl (MOPC-21), PE anti-human MICA/MICB (6D4), PE anti-human CD336 (NKp44) (P44-8), PE anti-human CD337 (NKp30) (P30-15), and PE anti-human CD16 (3G8) (BioLegend, Inc., San Diego, CA, USA), and Human ULBP-1 PE-conjugated Antibody (170818), Human ULBP-2/5/6 PE-conjugated Antibody (165903), and Human ULBP-3 PE-conjugated Antibody (166510) (R&D System, Minneapolis, MN, USA). .. The YSH-LV001 vector (Ubigene Biosciences, Guangzhou City, China) was used to construct a specific CD56 shRNA-expressing plasmid.



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    Cytokine secretion, cytolytic activity and proliferation of CD19/NKG2DL tandem CAR T-cells co-cultured with CD19+ and CD19- Nalm-6 cells. (A) Expression of CD19 and NKG2DL in Nalm-6 and Nalm-6 CD19 KO cells. (B) Expression of individual NKG2DL using antibodies directed against MICA, MICB, <t>ULBP1,</t> ULBP3 or ULBP2-5-6 (grey histograms) vs autofluorescence (white histograms). (C) Secretion of IFN-γ, TNF-α and IL-2 cytokines after a 24-hour co-culture at 1:1 E:T ratio with Nalm-6 and Nalm-6 CD19 KO cells. (D) Cytolytic activity of CAR T-cells against Nalm-6 and Nalm-6 CD19 KO cells at 1:1 and 0.1:1 E:T ratio. Results are expressed as percentage of remaining cancer cells normalized to t0h timepoint. (E) Representative experiment showing CTV histograms of CAR T-cells after 4 days of co-culture without cancer cells or at 1:1 E:T ratio with Nalm-6 and Nalm-6 CD19 KO cells (4 individual experiments were performed on 4 different donors). Adjusted P values (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001) were determined by one-way ANOVA with Dunnet’s correction for multiple comparisons. Data presented as means (SD) of n=5. Each symbol denotes a different PBMC donor.
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    Image Search Results


    Cytokine secretion, cytolytic activity and proliferation of CD19/NKG2DL tandem CAR T-cells co-cultured with CD19+ and CD19- Nalm-6 cells. (A) Expression of CD19 and NKG2DL in Nalm-6 and Nalm-6 CD19 KO cells. (B) Expression of individual NKG2DL using antibodies directed against MICA, MICB, ULBP1, ULBP3 or ULBP2-5-6 (grey histograms) vs autofluorescence (white histograms). (C) Secretion of IFN-γ, TNF-α and IL-2 cytokines after a 24-hour co-culture at 1:1 E:T ratio with Nalm-6 and Nalm-6 CD19 KO cells. (D) Cytolytic activity of CAR T-cells against Nalm-6 and Nalm-6 CD19 KO cells at 1:1 and 0.1:1 E:T ratio. Results are expressed as percentage of remaining cancer cells normalized to t0h timepoint. (E) Representative experiment showing CTV histograms of CAR T-cells after 4 days of co-culture without cancer cells or at 1:1 E:T ratio with Nalm-6 and Nalm-6 CD19 KO cells (4 individual experiments were performed on 4 different donors). Adjusted P values (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001) were determined by one-way ANOVA with Dunnet’s correction for multiple comparisons. Data presented as means (SD) of n=5. Each symbol denotes a different PBMC donor.

    Journal: Frontiers in Immunology

    Article Title: Tandem CAR T-cells targeting CD19 and NKG2DL can overcome CD19 antigen escape in B-ALL

    doi: 10.3389/fimmu.2025.1557405

    Figure Lengend Snippet: Cytokine secretion, cytolytic activity and proliferation of CD19/NKG2DL tandem CAR T-cells co-cultured with CD19+ and CD19- Nalm-6 cells. (A) Expression of CD19 and NKG2DL in Nalm-6 and Nalm-6 CD19 KO cells. (B) Expression of individual NKG2DL using antibodies directed against MICA, MICB, ULBP1, ULBP3 or ULBP2-5-6 (grey histograms) vs autofluorescence (white histograms). (C) Secretion of IFN-γ, TNF-α and IL-2 cytokines after a 24-hour co-culture at 1:1 E:T ratio with Nalm-6 and Nalm-6 CD19 KO cells. (D) Cytolytic activity of CAR T-cells against Nalm-6 and Nalm-6 CD19 KO cells at 1:1 and 0.1:1 E:T ratio. Results are expressed as percentage of remaining cancer cells normalized to t0h timepoint. (E) Representative experiment showing CTV histograms of CAR T-cells after 4 days of co-culture without cancer cells or at 1:1 E:T ratio with Nalm-6 and Nalm-6 CD19 KO cells (4 individual experiments were performed on 4 different donors). Adjusted P values (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001) were determined by one-way ANOVA with Dunnet’s correction for multiple comparisons. Data presented as means (SD) of n=5. Each symbol denotes a different PBMC donor.

    Article Snippet: NKG2DL were detected using antibodies directed against human MICA (FAB1300P, R&D systems), MICB (FAB1599P, R&D systems), ULBP1 (FAB1380P, R&D systems), ULBP3 (FAB1517P, R&D systems), ULBP2-5-6 (FAB1298P, R&D systems) or using a recombinant human NKG2D (rhNKG2D-Fc, 1299-NK-050, R&D systems) detected with an anti-human IgG-Fc antibody (12-4998-82, ThermoFisher).

    Techniques: Activity Assay, Cell Culture, Expressing, Co-Culture Assay